Third and fourth instars of Rhodnius neglectus and Triatoma infestans were fed on wild opossums (Didelphis) with natural infeccions by Trypanosoma cruzi and, examined at regular 3.5 days intervals. Best results were obtained with rectum examinations and the infeccion was more easily detected from the 15th to the 20th days. R. neglectus showed better performance than T. infestans and so its utilisation in the xenodiagnosis technique is suggested.

Although there has been an improvement in the diagnosis of chronic Chagas’ disease, the low sensitivity of indirect parasitological tests is a drawback to its application in diagnosis and post-therapeutic control. Polymerase chain reaction (PCR) has limited use in routine diagnosis due to the need of specific laboratory facilities, common DNA cross-contamination, and high costs. At the same time, the high variability of PCR results found in different regions of Brazil raises some questions concerning its applicability for diagnosis. PCR’s […]